Double-Locus Sequence Typing Using clfB and spa , a Fast and Simple Method for Epidemiological Typing of Methicillin-Resistant Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Double-locus sequence typing using clfB and spa, a fast and simple method for epidemiological typing of methicillin-resistant Staphylococcus aureus.
Sequence-based epidemiological typing of methicillin-resistant Staphylococcus aureus (MRSA) has recently been promoted because it results in unambiguous data sets that can be organized in local and global databases. The replacement of previous typing methods, such as the highly discriminatory pulsed-field gel electrophoresis (PFGE), has been attempted with various markers and typing schemes, in...
متن کامل[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].
OBJECTIVE To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA. METHODS Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the alle...
متن کاملEvaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis.
OBJECTIVE spa-typing of methicillin-resistant Staphylococcus aureus (MRSA) has been used widely in clinical diagnostics and epidemiological studies. The aim of this study was to evaluate high-resolution melting (HRM) as a rapid and cost-effective method, to replace DNA-sequencing, for spa-typing in a global collection of 50 MRSA isolates. METHODS The polymorphic X region of the spa gene was a...
متن کاملMultilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus.
A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155 S. aureus isolates from patients with community-acquired and hospital-acquired invasive disease in the Oxford, United Kingdom, area. Fifty-three different allelic profiles were identified, and 17 of these were represented ...
متن کاملspa Typing of Staphylococcus aureus as a frontline tool in epidemiological typing.
We determined the value of spa typing in combination with BURP (based upon repeat pattern) grouping analysis as a frontline tool in the epidemiological typing of Staphylococcus aureus, based on a random collection of 1,459 clinical isolates sent to the German Reference Centre for Staphylococci within a 6-month period. The application was found to be helpful for the classification of isolates in...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2007
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00121-07